enzyme linked immunosorbent assay elisa  (Elabscience Biotechnology)

Journal: Frontiers in Endocrinology

Article Title: Effects of different sugar-lipid ratio diets on the occurrence of type 2 diabetes mellitus

doi: 10.3389/fendo.2026.1768263

Figure Lengend Snippet: Insulin resistance and metabolic profile of mice treated with different carbohydrate-fat ratio diets. (A) Fasting serum insulin levels(FINS) in mice (n = 6). (B) Homeostasis Model Assessment-Insulin resistance (HOMA-IR) in mice (n = 6). (C) Triglyceride glucose (TyG) index in mice (n = 6). (D–G) Serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density cholesterol (HDL-C) levels (n = 6). (H) Serum adiponectin levels in mice (n = 6). (I) Serum leptin levels in mice (n = 6). (J) Serum TNF-α levels in mice (n = 6). (K) Serum IL-1β levels in mice (n = 6). (L) Serum IL-6 levels in mice (n = 6). In statistical analysis, all values are expressed as mean ± SD. In statistical analysis, all values are expressed as mean ± SD. * indicates statistical difference between the model and control groups, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Additionally, serum levels of adiponectin (ED295; Lianke Bio, China) and leptin (EK-297; Lianke Bio, China) were analyzed using their respective ELISA kits.

Techniques: Control

Journal: The FASEB Journal

Article Title: Lymphocyte Antigen 6G Mediates Vagotomy‐Associated Reduction in Body Weight

doi: 10.1096/fj.202600151RR

Figure Lengend Snippet: Vagotomy promoted Ly6G + cell infiltration into eWAT. Wild‐type mice were subjected to left cervical vagotomy (VX) or sham surgery and tissues were collected at 7 days following surgery. (A) The percentage of non‐adipocyte nuclei of total nuclei per section was quantified using ImageJ ( n = 4) and right panels show representative images of paraffin sections of eWAT stained with H&E in sham and VX animals. (B) CCL2 release from eWAT was analyzed by ELISA. The bar shows the CCL2 levels from sham ( n = 4) or VX ( n = 4) mice normalized to eWAT weight: ng/mL per g ± SEM (unpaired Student's t test). (C) eWAT was collected at 1 ( n = 3), 4 ( n = 4 sham, n = 5 VX), and 7 ( n = 15) days following VX or sham surgery and the eWAT SVCs were analyzed by flow cytometry. The bar shows the % ± SEM of CD11b + Ly6G + cells from CD45 + (one‐way ANOVA, Uncorrected Fisher's LSD). (D) Graphs show representative gating for CD11b + Ly6G + cells in sham and VX eWAT at 7 days (concatenated n = 5–6). (E) Representative immunostaining of Ly6G (red) and Perilipin1 (green) in paraffin sections of eWAT. (F–H) Bone marrow neutrophils after sham ( n = 9) or VX ( n = 5) surgery were isolated using negative magnetic beads and analyzed using bulk RNAseq (DESeq2). Heatmap (F), volcano plot (G) of differentially expressed genes, and GO (Gene Ontology) (H) enrichment bar plot. ns = not significant, * p < 0.05. VX, Vagotomy; eWAT, epididymal white adipose tissue; H&E, hematoxylin–eosin; SVCs, stromal vascular cells.

Article Snippet: In vitro release rate was calculated as mg NEFAs per mg eWAT tissue per hour. (2) CCL2 levels were quantified using a mouse CCL2 ELISA kit (R&D Systems, #DY497‐05), according to the manufacturer's instructions ( n = 1 experiment).

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunostaining, Isolation, Magnetic Beads, RNA sequencing